Journal: Viruses

Article Title: The Junction Between nsp1β and nsp2 in the Porcine Reproductive and Respiratory Syndrome Virus Genome Is a New Site for the Insertion and Expression of Foreign Genes.

doi: 10.3390/v17050656

Figure Lengend Snippet: Figure 4. Fluorescent focus unit reduction-based assay for detection of neutralizing antibodies against PRRSV. The fluorescent focus unit reduction-based assay was performed using rPRRSV-SH01-eGFP at a dose of 100 TCID50 and serum samples diluted from 1:4 to 1:64. The fluorescent foci were captured using a multi-detector microplate reader at 48 h post-inoculation. (A) Green fluorescence of the tested samples was visualized under an inverted fluorescence microscope. Scale bar: 200 µm. (B) Fluorescent focus units of the serum samples from vaccinated pigs. (C) Fluorescent focus units of the serum samples from naïve pigs. Data are expressed as the mean ± standard deviation (SD). V, cells infected with rPRRSV-SH01-eGFP alone as a virus control; M, mock-infected cells as a negative control.

Article Snippet: The fluorescence signal of each well was read on a multi-detector microplate reader (SpectraMax M3; Molecular Devices, San Jose, CA, USA) at Ex/Em = 485/535 nm at 48 h post-inoculation.

Techniques: Microscopy, Standard Deviation, Infection, Virus, Control, Negative Control